Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals ü. Vacuolar Localization of Some Hydrolases

In vacuoles isolated from petal upper epidermis o f Petunia hybrida through polybase-induced lysis of protoplasts the vacuolar localization o f x-mannosidase and x-galactosidase was dem on­ strated. No association was observed between the tonoplast and these hydrolases. Protoplasts from different mutant lines blocked in anthocyanin biosynthesis were treated with DEAE-dextran to release vacuoles. The differences in quality o f the obtained vacuolar preparations indicated the relation between mutant line used and successful application of the polybase-procedure.